Primer
Prefix-R

Part:BBa_G1001

Designed by: Jason Kelly   Group: iGEM06_MIT   (2008-04-22)


Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

This is the REV primer used in the measurement kit to prepare pSB3K3

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 17
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 17
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//primer/plasmid/amplification
Parameters
directionR
len_end
len_fr
len_start
pct_gc59.1
targetBioBrick plasmid backbones
t_m60.3